Whole Transcriptome Amplification Services
Important factors about input RNA
- Any RNA source can be used
- Molecular weight should be more than 300 bases
- RNA can be isolated using standard methods or kits
- RNA can be single-stranded or double-stranded
- For most robust performance input should be at least 50 ng of RNA at a concentration of >5 ng/uL in TE
- Samples < 5 ng RNA are useable
Important features of WTA amplified cDNA
- WTA is very reproducible—Up to 99% microarray data correlation between independent amplifications.
- Human, animal, plant, microbial and viral RNA amplify similarly.
- Coding and non-coding RNA amplify similarly.
- Polyadenylated and non-adenylated RNA amplify similarly.
- No 3’ bias
- WTA yields 10 ug – 200 ug amplified cDNA without compromise of quality
- Average molecular weight ~ 400 b
- Natural base composition
- dsDNA, with variable amount of ssDNA
- Virtually no background
- Uniform concentration of ~100 ng/uL
- Virtually free of dNTPs and oligonucleotide primers
- Nuclease-free
- PCR inhibitor free
- Stable at -20*C
Example project specifications and cost
- Normally more than twenty samples are grouped into a project.
- Input RNA—50 ng at > 5 ng/uL in TE or water, 96-well format with 4 unused control wells
- Output cDNA—Multiples of 10 ug at >100 ng/uL in TE, purified by Millipore Montage, 96-well format
- Input and output nucleic acid is subject to QC procedures so that the customer receives uniform product quality and concentration. If input QC criteria of DNA quantity and concentration are met, Rubicon guarantees amplification to a specific amount of PCR-amplifiable cDNA.
- Turn-around—Normally 2 - 4 weeks, subject to production schedule
- Quantity discounts available
Value-added services
- Extraction of RNA from frozen or fixed tissue
- Preparation of array-ready labeled WTA cDNA as 25 – 100 b fragments with biotin or fluorescent labels.
- Custom amplification with “GC-boost” reagents to normalize amplification of extremely GC-rich sequences.
- Proofreading available on request.
- Contract quantitative Q-PCR expression service
Project flow
Think about your requirements, such as:
- Number and type of samples
- Amount of WTA cDNA required
- Importance of specific quality control measures
- Your timeline
Based on your requirements, Rubicon will quickly generate a project quote that contains all information about how your needs will be addressed, how to ship the samples to Rubicon, and contact information for all production matters. Once you approve of the project scope and quote, you will be asked for a Purchase Order or credit card number. When the Purchase Order or payment is received, and the samples are received by Rubicon, the project goes into production.
Typically, a Material Transfer Agreement is not necessary for an academic or institutional project. Proprietary samples sometimes require an MTA or other appropriate document, which Rubicon can provide.
Rubicon requires that you have IRB approval for your project, but does not need any evidence supporting that in our files. No patient data (coded or otherwise) should be sent with the samples.
Rubicon supports you with a direct contact in Commercial Operations, an electronic report that accompanies the return of amplified cDNA, and continuing access to expert scientists and management.
Customer Support and New Projects
To discuss existing projects, customers should contact Dr. Takao Kurihara, Associate Director of Commercial Operations, through our contact form or by phone at (734) 677-6894.
To discuss new projects, new and existing customers should contact Dr. John Langmore, VP Commercial Development, through our contact form or by phone at (734) 677-2797.
To discuss possible collaborations and partnerships, please contact Dr. John Langmore, VP Commercial Development, through our contact form or by phone at (734) 677-2797.
Files
More information is available in the following downloadable files: