OmniPLEX Family of Products

Commercial Products

TransPLEX C-WTA Kit (Cat. # RC20050)
- For Whole Transcriptome Amplification (Diagnostic Applications only)
- 50 Reactions Kit
- Offered through Rubicon Genomics.

TransPLEX WTA Kit (Research Applications - Sigma Aldrich)
- For Whole Transcriptome Amplification
- Licensed to Sigma Aldrich for the manufacturing and distribution to the research market

GenomePLEX WTA Kit (Research Applications - Sigma Aldrich)
- For Whole Genome Amplification
- Licensed to Sigma Aldrich for the manufacturing and distribution to the research market

Attributes and Features

High DNA yields from difficult or denatured samples
Simple 1 tube, 5 step fast protocol that's suitable for automation
Leading choice for FFPE expression and chromosomal array-based comparative genomic hybridization profiling
Designed to work with nucleic acid inputs ranging from 1 ng to 300 ng
Excellent performance with archived FFPE samples

Background

Proven reliability and performance since 2003 commercialization
Amplification incorporates a denaturation first step that enables this chemistry to work well with ds or ss DNA or RNA
First Rubicon kit manufactured under cGMP
OmniPLEX kit for research applications available from Sigma Aldrich; kits for commercial applications are available directly from Rubicon Genomics

Publications Highlighting OmniPLEX

Kim et al. (2011) Genome Res. 2011:1028-1041	Deep sequencing identifies distinct methylation patterns during cancer progression
Gonzalez-Roca et al. (2010) PLoS ONE 5(12):e14418.	Accurate Expression Profiling of Very Small Cell Populations. Accurate expression profiling from as few as 10 cells using the Sigma TransPlex WGA2 kits
Beck et al. (2010) Mol Cancer Res 8(3):335-342.	Next Generation Sequencing of Serum Circulating Nucleic Acids from Patients with Invasive Ductile Breast Cancer Reveals Differences to Healthy and Nonmalignant Controls. Use of GenomePlex WGA to amplify circulating DNA for sequencing on the 454/Roche GS-FLX platform