Rubicon Genomics

ThruPLEX Family of Products

Commercial Products

ThruPLEX-FD Prep Kit (Cat.# R40048 48rxn, # R40012 12rxn)
         - Single tube sample preparation for Illumina® NGS Platforms for Nanogram to Picogram Inputs of Fragmented dsDNA
         - 48 Reactions Kit and 12 Reactions Kit

Attributes and Features

• Developed specifically for NGS applications
• Protocols designed for use with difficult samples: plasma, FFPE, cDNA, and ChIP DNA
• Patented technology delivers high performance from sub-nanogram inputs of DNA
• Single tube design results in less contaminants, cleaner preps yielding lower backgrounds and better sequencing data,
• Fewer processing steps, shorter incubation times results in greater daily output
• Stable reagents and simple fast protocols enabling automation

• ThruPLEX was developed to meet the requirements of the expanding genetic testing market, namely:
  • Molecular analysis of clinical samples needs to be more sensitive and more rapid than analysis of research samples
  • NGS genetic testing will become the method of choice for existing and new applications
  • Genetic testing has to adapt to using less amounts of sample while increasing sensitivities
  • Testing must work on a range of challenging sample types
• ThruPLEX design is novel and proprietary
  • Optimized mixtures of  oligonucleotides, cofactors and enzymes
  • An efficient ligation-based NGS amplification format
• Evaluated by independent industry-leading laboratories:
  • Product meets its claims
  • Product outperforms existing commercial tests
  • Validated on HiSeq with sub-nanogram inputs of plasma, FFPE, ChIP, and fragmented DNA samples
• Click ThruPLEX Schematic to view a schematic representation of the base Stem-Loop Adaptor chemistry approach

Publications Highlighting ThruPLEX-FD