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TransPlex C-WTA for fixed and other degraded RNA

Reproducible Whole Transcriptome Amplification from Fixed and Other Degraded Samples

TransPlex C-WTA Applications:

  • Gene expression profiling from biopsies and surgical tissue
  • Biomarker testing of archived FFPE sections
  • Splice variant analysis

TransPlex™ C-WTA is based on special enzymes and random-priming oligonucleotides to make and amplify a library of short, overlapping amplimers that are a very faithful representation of total cellular RNA. TransPlex yields excellent results with fragmented RNA greater than 400 b long even if containing lesions. It is a very effective method to amplify RNA damaged from formalin fixation, radiation, or shearing. TransPlex is not recommended for less than 100 analyte copies because of reduced reproducibility of the analysis.

picoplex workflow TransPlex Workflow

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