WMA for profiling of methylation FFPE tissue or plasma
MethylPlex WMA services for enzymatic preparation of DNA for methylation analysis
We offer pre-analytical and analytical DNA MethylPlex methylation services to pharmaceutical companies, diagnostic companies and research institutions.
MethylPlex is our proprietary method for enzymatically digesting unmethylated CpG islands and selectively amplifying just the methylated CpG islands for study of gene regulation and discovery of biomarkers. For NGS and array applications, CpG islands can be enriched in order to increase analytical specificity and sensitivity.
Features of the Service
- Plasma, serum, and fixed or unfixed tissue samples
- Enables array, and NGS copy-number methylation profiling
- Used for highly sensitive biomarker discovery
- SYBRgreen Q-PCR sensitive to less than 0.01% methylation
- Arrays sensitive to < 3% methylation
- Bisulfite conversion not used, MSP and pyrosequencing unnecessary
- 96-well format
- As little as 1,000 cells needed for excellent results
Important features of amplified bisulfite-converted DNA
- Compatible with MSP, pyrosequencing, array and NGS analysis
- Products compatible with enrichment for NGS
- Superior representation of even GC-rich loci with minimal allele bias
- Virtually no background from unmethylated CpG islands
- Validated on thousands of tissue and plasma samples.
- Stable at -20*C for years
Value-added services
- Extraction of DNA from plasma, serum, tissue or FFPE section
- Design or performance of MethylPlex Q-PCR assays.
- Biomarker discovery and validation services
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