WGA and WTA from FFPE Sections

Our WGA and WTA Services for FFPE DNA give you the ultimate benefit from our GenomePlex, PicoPlex and TransPlex platforms coupled with the expertise of their developers.

We have successfully completed projects using every source of nucleic acid and application known to genomics and use this experience to adjust protocols, if necessary, to provide a superior product on a fixed schedule. Rigorous quality control insures the best results every time.

Important factors about input genomic DNA/RNA

  • Human, animal, plant or microbial DNA or RNA
  • Molecular weight should be more than 200 bases
  • DNA/RNA can be in solution or formalin-fixed paraffin sections
  • DNA/RNA can be isolated using any of the standard methods or kits
  • DNA/RNA can be single-stranded or double-stranded

Important features of amplified DNA or cDNA

  • Mixture of ds and ss DNA, MW ~ 500 b
  • 3 – 10 micrograms DNA, ~50 micrograms/microliter
  • Virtually no background (negative controls show no DNA amplification)
  • Excellent representation of all sequences including GC-rich DNA
  • Products compatible with enrichment for NGS
  • Stable at -20*C for years

Value-added services

  • Extraction of DNA or RNA from FFPE tissue

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