breaking the sample barrier

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Publications

 PicoPLEX               TransPLEX               ThruPLEX              Tissue              Single Cell               Formalin Fixed               Fragmented


 

PicoPLEX
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2012

First randomised prospective IVF study of pre-implantation chromosome analysis using 24sure aCGH system (with SurePlex WGA) demonstrating that selectively implanting euploid embryos significantly increases pregnancy rates in young patients

NGS analysis of single bacteria cells using PicoPlex amplification on microfluidic device

Oligo aCGH analysis of PicoPlex-amplified single human cells to detect genomic inbalances with CNVs as small as 1.2 Mb

2011

Completely phased genome sequencing through chromosome sorting. NGS analysis of PicoPlex-amplified single-copy sorted chromosomes to infer the haplotype of individual human chromosomes.

Investigation of aneuploidy related to age by aCGH screening of porcine oocytes amplified by PicoPlex

2010

Validation of microarray comparative genomic hybridization for comprehensive chromosome analysis of embryos.

SNP microarray-based 24 chromosome aneuploidy screening demonstrates that cleavage-stage FISH poorly predicts aneuploidy in embryos that develop to morphologically normal blastocysts.

Omics in assisted reproduction: Possibilities and pitfalls.

SNP microarray-based 24 chromosome aneuploidy screening is significantly more consistent than FISH.

2007

Whole genome amplification from a single cell: a new era for preimplantation genetic diagnosis.

 

TransPLEX 
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2011

This is a milestone paper using the Rubicon OmniPLEX prep for methylation profiling by next-generation sequencing

2010

Use of GenomePlex WGA of serum to detect breast and prostate cancer using massively-parallel sequencing.

Accurate expression profiling from as few as 10 cells using the Sigma TransPlex WGA2 kits

Use of GenomePlex WGA to amplify circulating DNA for sequencing on the 454/Roche GS-FLX platform.

Use of GenomePlex WGA to detect multiple sclerosis from serum.

2009

GenomePlex WGA used to karyotype, genotype, and sequence single human blastomeres.

Use of GenomePlex WGA of serum DNA for early detection of BSE using Roche/454 GS-FLx.

GenomePlex WGA used for deep sequencing of ChIP DNA

2008

GenomePlex gave superior results for mutation detection in FFPE tissue.

GenomePlex used to increase the success rates of PGD using STR and mutation assays.

Use of TransPlex for discovery of expression biomarkers in urine.

GenomePlex gave superior genotyping results for amplifying DNA from degraded DNA.

2007

 

GenomePlex WGA and TransPlex WTA was used to amplify cDNA to enable rapid determination of viral RNA sequences.

 

 

 

 

 

Shows that GenomePlex can be used for single-cell CGH.

Single-cell array CGH using GenomePlex WGA.

Shows that GenomePlex WGA of hair can be used for SNP genotyping

GenomePlex WGA gives excellent oligonucleotide array CGH results.

GenomePlex was validated for genotyping of DNA extracted from Guthrie cards.

GenomePlex validated for unbiased amplification of bacterial DNA for microarray analysis.

2006

 

 

 

GenomePlex WGA for large SNP study on Illumina platform.

Superior results with GenomePlex WGA for FFPE material.

Adopted GenomePlex as superior to ligation-mediated PCR because of higher S/N ratio.

2005

GenomePlex amplimers are too small to genotype using a specific set of forensic STRs. (In research studies GenomePlex DNA produced excellent genotyping using the complete well-known Marshfield STR panel).

2004

GSK and Illumina study shows 99.8% SNP genotype concordance between GenomePlex amplified DNA and unamplified human genomic DNA.

GenomePlex WGA from single-copy sorted chromosomes gives excellent array CGH.

 

GenomePlex WGA of individual sorted chromosomes can synthesize excellent FISH probes for CGH.

 

ThruPLEX 
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2013

 

2012

ThruPLEX Used in Non-Invasive Fetal WGS

2010

New genetic associations detected in a host response study to hepatitis B vaccine.

 

Tissue
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2011

This is a milestone paper using the Rubicon OmniPLEX prep for methylation profiling by next-generation sequencing.

2010

TransPlex WTA was used for microarray expression studies of 5,000 – 10,000 microdissected prostate cancer cells.

2007

GenomePlex WGA and TransPlex WTA was used to amplify cDNA to enable rapid determination of viral RNA sequences.

 

Shows that GenomePlex WGA of hair can be used for SNP genotyping

GenomePlex WGA gives excellent oligonucleotide array CGH results.

GenomePlex was validated for genotyping of DNA extracted from Guthrie cards.

 

GenomePlex validated for unbiased amplification of bacterial DNA for microarray analysis.

2006

GenomePlex WGA for large SNP study on Illumina platform.

GSK and Illumina study shows 99.8% SNP genotype concordance between GenomePlex amplified DNA and unamplified human genomic DNA.

 

Single Cell
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2011

The Rubicon PicoPlex™ WGA (distributed under the trademark SurePlex™ by BlueGnome) was used to amplify single human blastomeres for microarray detection of chromosome translocations before implantation. The results show improved accuracy of aneuploidy and reciprocal translocation detection compared to FISH-based testing.

In the porcine model organism, PicoPlex WGA was shown to improve detection of aneuploidy of oocytes compared to FISH testing.

Very good review of the methods and medical applications of single cell sequencing, including using GenomePlex WGA for next-gen sequencing of single cells.

GenomePlex WGA amplification of single human nuclei was used to accurately determine copy number variations in tumor cell populations. This was used to reconstruct evolution of a heterogeneous population of cancer cells.

This landmark study concluded that PicoPlex WGA (distributed as SurePlex by BlueGnome) had almost 4 times fewer analysis failures and almost 5 times fewer misdiagnoses than GenomePlex WGA (distributed by Sigma-Aldrich).

This landmark study documents the first births after PGD of chromosomal abnormalities using single cell aCGH, which used the Rubicon PicoPlex WGA process (distributed as SurePlex by BlueGnome).

PicoPlex WGA was used with a home-brew method of adding NGS adaptor sequences to sequence single-copy sorted chromosomes to infer the haplotype of individual human chromosomes.

2010

 

GenomePlex WGA was used to amplify small numbers of cancer cells for array CGH

GenomePlex WGA is used to amplify small numbers of tumor cells for genotyping to understand tumor progression.

Accurate expression profiling from as few as 10 cells using the Sigma TransPlex WGA2 kits

 

 

2009

GenomePlex WGA used to karyotype, genotype, and sequence single human blastomeres.

2008

GenomePlex used to increase the success rates of PGD using STR and mutation assays.

2007

 

Shows that GenomePlex can be used for single-cell CGH.

Single-cell array CGH using GenomePlex WGA.

 

2004

GenomePlex WGA from single-copy sorted chromosomes gives excellent array CGH.

GenomePlex WGA of individual sorted chromosomes can synthesize excellent FISH probes for CGH.

 

Formalin Fixed
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2008

GenomePlex gave superior results for mutation detection in FFPE tissue.

2006

GenomePlex WGA was used for successful array CGH of as few as 2,000 tumor cells microdissected from FFPE tissue, and from 10 - 20 ng of DNA from FFPE samples.

 

Superior results with GenomePlex WGA for FFPE material.

 

Fragmented 
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2009

GenomePlex WGA used for deep sequencing of ChIP DNA

2008

GenomePlex gave superior genotyping results for amplifying DNA from degraded DNA.

2007

 

2006

Adopted GenomePlex as superior to ligation-mediated PCR because of higher S/N ratio.