TransPlex™ C-WTA for Fixed and Other Degraded RNA

Reproducible Whole Transcriptome Amplification from Fixed and Other Degraded Samples
TransPlex™ C-WTA does an excellent job of amplifying total RNA from intact or degraded samples, giving you:

• The ability to study gene expression using biopsies and surgical tissue
• Biomarker discovery from archived FFPE sections
• Expression profiling of fixed FACS samples
• Splice variant analysis

Superior Performance

• Highly representative amplification of total RNA
• Effective for RNA fragments above 300 bases
• 90% sample success for archived FFPE collections

Simple, Rapid Protocol

• 3-hour, single-tube, two-step process
• No intermediate purifications, no final purification necessary
• Completely automatable
• Requires 100 – 300 ng RNA for optimal results

Important Features of TransPlex Amplified DNA

• Mixture of ds and ss DNA, MW ~ 500 b
• 3 – 5 micrograms DNA, ~50 micrograms/microliter
• Virtually no primer dimers, chimeras, or extraneous DNA background
• Excellent representation of even highly GC-rich sequences.
• Used on thousands of sections to discover diagnostic and prognostic markers

The TransPlex C-WGA kits amplify 50 samples for microarray, Q-PCR or NGS analysis. TransPlex C-WTA is a General Purpose Reagent (21CFR86) intended to be used before clinical testing of human, animal, or plant samples. Researchers not testing clinical samples must purchase the Sigma-Aldrich TransPlex WTA kit.

Please contact Rubicon for more information.