IP-Plex WGA for Immunoprecipitates
Reproducible Whole Genome Amplification from small amounts of sonicated DNA including ChIP and meDIP preparations
IP-Plex™ WGA has built-in repair reactions to more efficiently amplify sonicated DNA, giving you:
- More sensitive array and NGS mapping of DNA binding proteins
- More sensitive array and NGS mapping of methylated genomic sequences
- The ability to amplify smaller fragments, which increases spatial resolution.
Superior Performance
- Effective amplification of ds-DNA between 100 – 2,000 bp in length
- High representation from even extremely GC-rich loci
- More sensitive and representative than conventional LM-PCR
- >10X more sensitive than the most popular method for ChIP WGA
Simple, Rapid Protocol
- Single-tube, 2-step, 3-hour protocol
- Integrated repair of DNA sonication damage
- No intermediate purifications, no final purification necessary
- Completely automatable
Important Features of IP-Plex Amplified DNA
- High sequence fidelity
- Molecular weight same as template DNA
- Virtually no primer dimers, chimeras, or extraneous DNA background
- Validated for use on Agilent and NimbleGen genomic, promoter, and CpG island arrays.
The IP-Plex WGA kits amplify 50 sonicated samples for microarray, Q-PCR or NGS analysis.
The IP-Plex WGA Kit is in development. For early access, please contact Rubicon.