PicoPLEX Family of Products
Commercial Products
PicoPLEX WGA Kit (Cat. # R30050)
- For Single-Cell Whole Genome Amplification
- 50 Reactions Kit
Attributes and Features
- Developed specifically for single cell and picogram quantities of DNA
- Designed for rapid, sensitive, and reproducible amplification
- Highly reproducible copy-number results
- Very low backgrounds
Background
- Products are designed for microarray or PCR analysis of single human cells launched in 2009
- The gold-standard for preparing single human cells for PGS microarray or PCR analysis
- Trade names: SurePLEX (BlueGnome); PicoPLEX WGA; Easy Amp (PerkinElmer); Single-Cell WGA (NEB)
- Used in ESHRE PGS clinical trials in 2011 and 2012
- Estimated ~90% of PGD/PGS cases in EU and US employ PicoPLEX WGA technology
Publications Highlighting PicoPLEX
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Yang et al. (2012) Mol. Cytogenetics 5:24 |
First randomised prospective IVF study of pre-implantation chromosome analysis using 24sure aCGH system (with SurePlex WGA) demonstrating that selectively implanting euploid embryos significantly increases pregnancy rates in young patients |
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Leung et al. (2012) PNAS 109(20):7665-7670 |
NGS analysis of single bacteria cells using PicoPlex amplification on microfluidic device |
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Bi et al. (2012) Prenatal Diagnosis 32:10-20 |
Oligo aCGH analysis of PicoPlex-amplified single human cells to detect genomic inbalances with CNVs as small as 1.2 Mb |
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Yang et al. (2011) PNAS 108(1):12-17 |
NGS analysis of PicoPlex-amplified single-copy sorted chromosomes to infer the haplotype of individual human chromosomes. |
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Hornak et al. (2011) PLoS One 6(4):e18892 |
Investigation of aneuploidy related to age by aCGH screening of porcine oocytes amplified by PicoPlex |